Recombinant Ovine Cytokines |
Estimated concentration (ng/ml) |
Estimated biological activity (U/ml) |
Bioassay |
References |
IL-1β |
25 |
In progress |
Induction of IL-8 release by ovine cells |
In preparation |
IL-2 |
40 |
Under titration |
Proliferation of ConA-activated ovine T cell blasts |
Deane et al (2000). J. Virol. 74,1313-1320. |
IL-3 |
500 |
Under titration |
Induction of colony growth in ovine bone marrow cultures in synergy with ovine stem cell factor |
McInnes et al (1993). Exp. Hematol. 21,1528-1534.
Macaldowie et al (1997). Vet. Immunol. Immunopathol. 60, 97-110;
McInnes et al (1999). Cytokine 11, 249-256. |
IL-4 |
ND |
100 |
Induction of intelectin expression by ovine tracheal explants |
Hope et al (2005). J. Immunol. Methods 301, 114-123;
French et al (2007). Vet. Immunol. Immunopathol. 120, 41-46. |
IL-5 |
ND |
100 |
Induction of eosinophil growth in ovine bone marrow cultures |
Stevenson et al (1998). Vet. Immunol. Immunopathol. 66, 359-365. |
IL-6 |
ND |
In progress |
In progress |
In preparation |
IL-8/CXCL8 |
100 |
In progress |
In progress |
In preparation |
IL-10 |
ND |
6500 |
Inhibition of ConA-induced proliferation of ovine T cells |
Kwong et al. (2002). Vet. Immunol. Immunopathol. 85, 213-223; Wattegedera et al (2004). Vet. Immunol. Immunopathol 102, 67-76. |
IL-12 |
ND |
3200 |
Induction of IFN-g release by ovine T cells |
Hope et al (2002). J. Immunol. Methods 266, 117-126;
Wattegedera et al (2004). Vet. Immunol. Immunopathol. 102, 67-76. |
GM-CSF |
20,000 |
100,000 |
Stimulation of granulocyte-macrophage colonies in ovine bone marrow cultures |
McInnes and Haig (1991). Gene 105, 275-279.
Entrican et al (1996). Vet. Immunol. Immunopathol. 50, 105-115;
McInnes et al (1999). Cytokine 11, 249-256.
Rooke et al (2005). Reprod. Fertil. Dev. 17, 513-521. |
IFN-g |
5 |
10,000 |
Inhibition of Semliki virus replication; Inhibition of chlamydial growth |
Graham et al (1995). J. Comp. Pathol. 112, 185-195.
Entrican et al (1998). Comp. Immunol. Microbiol. Infect. Dis. 21, 15-26. |
SCF |
ND |
Under titration |
Induction of colony growth in ovine bone marrow cultures in synergy with ovine IL-3 |
Macaldowie et al (1997). Vet. Immunol. Immunopathol. 60, 97-110;
McInnes et al (1999). Cytokine 11, 249-256. |
TNF-a |
100 |
50 |
Lysis of murine L929 cells; Induction of IL-8 release by ovine cells |
In preparation |
Recombinant Bovine Cytokines |
Estimated concentration
(ng/ml) |
Estimated biological activity (U/ml) |
Bioassay |
References |
IL-2 |
ND |
In progress |
Proliferation of ConA-activated bovine T cell blasts |
In preparation |
IL-4 |
70 |
Under titration |
Development of bovine dendritic cells in synergy with bovine GM-CSF |
Hope et al (2005) J. Immunol. Methods 301, 114-123.
|
IL-10 |
ND |
Under titration |
In preparation |
In preparation |
GM-CSF |
ND |
Under titration |
Development of bovine dendritic cells in synergy with bovine IL-4 |
In preparation |
IFN-aG |
ND |
5000 |
Inhibition of Semliki virus replication |
Chaplin et al (1996). J. Interferon. Cytokine Res. 16, 25-30. |
